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KMID : 0900919890130010018
Korean journal of Animal Reproduction
1989 Volume.13 No. 1 p.18 ~ p.25
Study on Motility and Acrosome Morphology of Fresh and Deep-frozen Korean Native Goat Spermatozoa




Park Chang-Sik
Abstract
This study was carried out to investigate the general semen characteristics of the Korean native goat and the effect of temperature, incubation time, dilution rate, freezing rate and glycerol concentration on motility and NAR (normal apical ridge) acrosome of fresh and frozen Korean native goat spermatozoa. 1. Average semen volume per ejaculate, motility, concentration and pH of fresh Korean native goat spermatozoa were 0.19{pm}0.09 ml, 94.5{pm}0.47%, 26.17{times}108{pm}1.68/ml and 6.63{pm}0.18, respectively. 2. Motility and NAR acrosome of fresh spermatozoa during incubation were higher at 22^{circ}C than at 5^{circ}C or 37^{circ}C(P<.01). 3. Motility and NAR acrosome of spermatozoa diluted 1:4 during incubation were higher at 22^{circ}C than at 5^{circ}C or 37^{circ}C(P<.01). 4. Motility and NAR acrosome of spermatozoa during incubation were higher for samples diluted 1:1, 1:2, or 1:4 than for samples diluted 1:6(P<.01). 5. Motility and NAR acrosome of post-thaw spermatozoa were higher at freezing rate of 12^{circ}C/min than at freezing rate of 1^{circ}C/min or 24^{circ}C/min when glycerol concentration was 9%(P<.01).
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